Annexin A2, an essential partner of the exocytotic process in chromaffin cells
نویسندگان
چکیده
منابع مشابه
Calcium, the cytoskeleton and calpactin (annexin II) in exocytotic secretion from adrenal chromaffin and mammary epithelial cells.
Introduction Exocytosis occurs in virtually all cell types; it can occur in a regulated fashion such that stored secretory vesicles only undergo exocytosis in response to changes in intracellular second messengers such as a rise in cytosolic free calcium concentration ([Ca2+],) or in a constitutive fashion immediately following vesicle biogenesis [ 11. A great deal is now known about the intrac...
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Annexin A2, a calcium-, actin-, and lipid-binding protein involved in exocytosis, mediates the formation of lipid microdomains required for the structural and spatial organization of fusion sites at the plasma membrane. To understand how annexin A2 promotes this membrane remodeling, the involvement of cortical actin filaments in lipid domain organization was investigated. 3D electron tomography...
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We have recently created a kinetic model that reproduces the dynamics of exocytosis with high accuracy. The reconstruction necessitated a search, in a multi-dimensional parameter space, for 37 parameters that described the system, with no assurance that the parameters, which reconstructed the observations, are a unique set. In the present study, a Genetic Algorithm (GA) was used for a thorough ...
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The exocytotic exposure of antigens of chromaffin granule membranes was studied with chromaffin cells isolated from bovine adrenal medulla. Antigens on the cell surface were visualized by indirect membrane immunofluorescence employing antisera against glycoprotein III and dopamine beta-hydroxylase. With unstimulated cells, only weak immunofluorescence on the cell surface was observed, whereas s...
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Annexin A2, a calcium-dependent phospholipid-binding protein, is abundantly expressed in alveolar type II cells where it plays a role in lung surfactant secretion. Nevertheless, little is known about the details of its cellular pathways. To identify annexin A2-regulated or associated proteins, we silenced endogenous annexin A2 expression in rat alveolar type II cells by RNA interference and ass...
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ژورنال
عنوان ژورنال: Journal of Neurochemistry
سال: 2016
ISSN: 0022-3042
DOI: 10.1111/jnc.13628