منابع مشابه
Optimization of Dynamic Binding Capacity of Anion Exchange Chromatography Media for Recombinant Erythropoietin Purification
Background:The dynamic binding capacity (DBC) of a chromatography matrix in protein purification is the amount of the total protein absorbed into the matrix, before occurrence of a significant break in the breakthrough curve. Optimization of the process criteria for maximum DBC avoids extra process scale-up and reduces ...
متن کاملoptimization of dynamic binding capacity of anion exchange chromatography media for recombinant erythropoietin purification
background:the dynamic binding capacity (dbc) of a chromatography matrix in protein purification is the amount of the total protein absorbed into the matrix, before occurrence of a significant break in the breakthrough curve. optimization of the process criteria for maximum dbc avoids extra process scale-up and reduces the processing time, costs and protein loss. taguchi method is a simple usef...
متن کاملAnion-Exchange Chromatography of Phosphopeptides: Weak Anion Exchange versus Strong Anion Exchange and Anion-Exchange Chromatography versus Electrostatic Repulsion–Hydrophilic Interaction Chromatography
Most phosphoproteomics experiments rely on prefractionation of tryptic digests before online liquid chromatography-mass spectrometry. This study compares the potential and limitations of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) and anion-exchange chromatography (AEX). At a pH higher than 5, phosphopeptides have two negative charges per residue and are well-retained...
متن کاملAnion/anion exchange in human neutrophils
Of the total one-way chloride fluxes (approximately 1.4 meq/liter cell water X min) in steady state human polymorphonuclear leukocytes bathed in 148 mM Cl media, approximately 70% behaves as self-exchange mediated by a nonselective anion carrier that is not inhibited by stilbene disulfonates. Five properties of this carrier-mediated exchange were investigated: substrate saturation is seen with ...
متن کاملAnion Exchangers: Ion Exchange
An economic comparison of reverse osmosis and ion exchange in Europe. In: Greig JA (ed.) Ion Exchange Development and Applications, pp. 58}66. Cambridge: Royal Society of Chemistry. Nolan J and Irving J (1984) The effect on the capacity of strong base anion exchange resins of the ratio of chloride to sulphate in the feed water. In: Naden D and Streat M (eds) Ion Exchange Technology, pp. 160}168...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Green Chemistry
سال: 2015
ISSN: 1463-9262,1463-9270
DOI: 10.1039/c5gc00828j