Anaplasma phagocytophilum Ecotype Analysis in Cattle from Great Britain

Tuberculosis in cattle in Great Britain.

Transformation of Anaplasma phagocytophilum

BACKGROUND Tick-borne pathogens cause emerging zoonoses, and include fastidious organisms such as Anaplasma phagocytophilum. Because of their obligate intracellular nature, methods for mutagenesis and transformation have not been available. RESULTS To facilitate genetic manipulation, we transformed A. phagocytophilum (Ap) to express a green fluorescent protein (GFP) with the Himar1 transposas...

Anaplasma phagocytophilum, Sardinia, Italy

1. McQuiston JH, Childs JE. Q fever in humans and animals in the United States. Vector Borne Zoonotic Dis. 2002;2: 179–91. 2. Maurin M, Raoult D. Q fever. Clin Microbiol Rev. 1999;12:518–53. 3. Stoker MGP, Marmion BP. The spread of Q fever from animals to man: the natural history of a rickettsial disease. Bull World Health Organ. 1955;13:781–806. 4. Spicer AJ. Military significance of Q fever: ...

On Anaplasma phagocytophilum in Horses

Equine Granulocytic Anaplasmosis (EGA) is an acute tick-borne infection caused by Anaplasma phagocytophilum. The bacterium can infect various animal species and humans. Persistence of infection is documented in ruminants and is shown experimentally in dogs, but it is unknown whether it occurs in horses or if EGA induces longer-term clinical changes in horses. This thesis includes results and an...

Anaplasma phagocytophilum from Rodents and Sheep, China

To characterize the strains of Anaplasma phagocytophilum in wild and domestic animals in China, we isolated the organism from rodents and sheep in northeastern China. We isolated 3 strains (2 from rodents and 1 from sick sheep) through propagation in BALB/c mice and then cell culture in HL60 cells. The 3 isolates were identified by Wright-Giemsa staining, immunofluorescence, and electronic micr...