Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe

Reactive oxygen species (ROS) play essential roles in intestinal homeostasis. ROS are natural by-products of cell metabolism. They produced response to infection or injury at the mucosal level as they involved antimicrobial responses and wound healing. also critical secondary messengers, regulating several pathways, including growth differentiation. On other hand, excessive levels lead oxidative stress, which can be deleterious for cells favor diseases like chronic inflammation cancer. This work provides a straightforward method detect murine organoids by live imaging flow cytometry, using commercially available fluorogenic probe. Here protocol describes assaying effect compounds that modulate redox balance specific types, exemplified here analysis stem genetically labeled with GFP. may used fluorescent probes.