Analytical validation of real-time quantitative PCR assays for optimum diagnosis of vivax malaria
نویسندگان
چکیده
منابع مشابه
Validation of Quantitative PCR Assays
can assess the safety of biotechnology products. Q-PCR precisely quantifies the amount of the nucleic acid target sequence in DNA or RNA extracted from a variety of samples including animal tissues, final products, cell banks, chromatography eluates, and bulk harvest material. During amplification with target-specific oligonucleotide primers, a fluorogenic oligonucleotide probe — with both repo...
متن کاملChecklist for optimization and validation of real-time PCR assays.
Real-time polymerase chain reaction (PCR) is a frequently used technique in molecular diagnostics. To date, practical guidelines for the complete process of optimization and validation of commercial and in-house developed molecular diagnostic methods are scare. Therefore, we propose a practical guiding principle for the optimization and validation of real-time PCR assays. Based on literature, e...
متن کاملComparison of Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Agglutination Assays in Diagnosis of Brucellosis in Golestan Province, North of Iran
Introduction: Brucellosis is one of the most common zoonotic infections worldwide. The clinical symptoms of brucellosis are similar to a wide range of diseases; hence, reliable diagnostic and laboratory methods are required to identify the causative agent. Iran is an endemic region of brucellosis, and many patients are misdiagnosed due to the nature of the infection. In this study, we aimed to ...
متن کاملDevelopment of Conventional and Real-Time Quantitative PCR Assays for Diagnosis and Monitoring of Scabies.
Scabies remains the most prevalent, endemic, and neglected ectoparasitic infestation globally and can cause institutional outbreaks. The sensitivity of routine microscopy for demonstration of Sarcoptes scabiei mites or eggs in skin scrapings is only about 50%. Except for three studies using conventional or two-tube nested PCR on a small number of cases, no systematic study has been performed to...
متن کاملValidation of a quantitative method for real time PCR kinetics.
Real time RT-PCR is the most sensitive method for quantitation of gene expression levels. The accuracy can be dependent on the mathematical model on which the quantitative methods are based. The generally accepted mathematical model assumes that amplification efficiencies are equal at the exponential phase of the reactions for the same amplicon. However, no methods are available to test the ass...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Memórias do Instituto Oswaldo Cruz
سال: 2019
ISSN: 1678-8060,0074-0276
DOI: 10.1590/0074-02760180350