Analytical Method Validation of Sulfamethoxazole in Human Plasma and Urine by HPLC-PDA

An analytical method for analyzing SMZ in human plasma and urine using high-performance liquid chromatography (HPLC) was developed. The separation carried out on a Shim-pack GIST® C18 column (150 × 4.6 mm, 5 ?m) with temperature setting of 30°C. mobile phase consisted glacial acetic acid pH 2.5: methanol: acetonitrile (70:25:5, v/v/v) at rate 0.8 mL/min. Detection done PDA detector the range ?190-800 nm, quantification ?265 nm. Plasma sample preparation used protein precipitation as precipitating solvent (1:3, v/v), while liquid-liquid extraction 0.03 M H2SO4 ethyl acetate. developed proven selective, linear (r = 0.998) 0.996) urine, accurate (%error ?11.76% LLOQ ?14.08% concentrations above), precision (%RSD ?4.52% ?4.48% sensitive 0.7 ?g/mL (plasma) 0.17 (urine). Stability tests were to determine shelf life samples under several conditions. is valid suitable pharmacokinetic studies urine.