Analysis of the Phospholipid Profile of Metaphase II Mouse Oocytes Undergoing Vitrification
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چکیده
منابع مشابه
Analysis of the Phospholipid Profile of Metaphase II Mouse Oocytes Undergoing Vitrification
Oocyte freezing confers thermal and chemical stress upon the oolemma and various other intracellular structures due to the formation of ice crystals. The lipid profiles of oocytes and embryos are closely associated with both, the degrees of their membrane fluidity, as well as the degree of chilling and freezing injuries that may occur during cryopreservation. In spite of the importance of lipid...
متن کاملP-102: Effect of Vitrification in Open Pulled Straw Vitrification of Live Birth Rates, Fertility and The Development of Mouse Oocytes at Metaphase II
Background: The cryopreservation of human oocyte would make significant contribution to fertility treatments. It could also provide on alternative to embryo preservation for avoidance ethical problems. Embryo cryopreservation is now a successful procedure, but oocyte cryopreservation has poorer results. Materials and Methods: For retrieval of oocyte NMRI mouse were used. after administration of...
متن کاملSlow freezing and vitrification differentially modify the gene expression profile of human metaphase II oocytes.
BACKGROUND Cryopreservation is now considered as an efficient way to store human oocytes to preserve fertility. However, little is known about the effects of this technology on oocyte gene expression. The aim of this study was to examine the effect of the two cryopreservation procedures, slow freezing and vitrification, on the gene expression profile of human metaphase II (MII) oocytes. METHO...
متن کاملHuman oocyte vitrification: the permeability of metaphase II oocytes to water and ethylene glycol and the appliance toward vitrification.
OBJECTIVE To determine the permeability of human metaphase II oocytes to ethylene glycol and water in the presence of ethylene glycol, and to use this information to develop a method to vitrify human oocytes. DESIGN An incomplete randomized block design. SETTING A university-affiliated assisted reproductive center. PATIENT(S) Women undergoing assisted reproduction in the Center for Reprod...
متن کاملOverexpression of Mitochondrial Genes (Mitochondrial Transcription Factor A and Cytochrome c Oxidase Subunit 1) in Mouse Metaphase II Oocytes following Vitrification via Cryotop
Background: Gamete cryopreservation is an inseparable part of assisted reproductive technology, and vitrification is an effective approach to the cryopreservation of oocytes. The aim of this study was to investigate vitrification effects on the expression levels of mitochondrial transcription factor A (Tfam) and mitochondrial-encoded cytochrome c oxidase subunit 1 (Cox1) in mouse metaphase II o...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2014
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0102620