Analysis of RNA base modification and structural rearrangement by single-molecule real-time detection of reverse transcription
نویسندگان
چکیده
منابع مشابه
Analysis of RNA base modification and structural rearrangement by single-molecule real-time detection of reverse transcription
BACKGROUND Zero-mode waveguides (ZMWs) are photonic nanostructures that create highly confined optical observation volumes, thereby allowing single-molecule-resolved biophysical studies at relatively high concentrations of fluorescent molecules. This principle has been successfully applied in single-molecule, real-time (SMRT®) DNA sequencing for the detection of DNA sequences and DNA base modif...
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Single-molecule analysis of RNA polymerase transcription.
The kinetics and mechanisms of transcription are now being investigated by a repertoire of single-molecule techniques, including optical and magnetic tweezers, high-sensitivity fluorescence techniques, and atomic force microscopy. Single-molecule techniques complement traditional biochemical and crystallographic approaches, are capable of detecting the motions and dynamics of individual RNAP mo...
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Rhinoviruses are the most frequent cause of human respiratory infections, and quantitative rhinovirus diagnostic tools are needed for clinical investigations. Although results obtained by real-time reverse-transcription PCR (RT-PCR) assays are frequently converted to viral RNA loads, this presents several limitations regarding accurate virus RNA quantification, particularly given the need to re...
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ژورنال
عنوان ژورنال: Journal of Nanobiotechnology
سال: 2013
ISSN: 1477-3155
DOI: 10.1186/1477-3155-11-8