Analysis of quaternary ammonium compounds and hexachlorophene by thin-layer chromatography and agar diffusion bioautography

Analysis of quaternary ammonium compounds and hexachlorophene by thin-layer chromatography and agar diffusion bioautography.

Bioautography detection in thin-layer chromatography.

Bioautography is a microbial detection method hyphenated with planar chromatography techniques. It is based mainly on antimicrobial or antifungal properties of analyzed substances. The review discusses three versions of bioautography, i.e. contact, immersion and direct bioautography. The more concern is given to the last one. Many applications are quoted, not only for testing various groups of ...

Rapid Identification of α-Glucosidase Inhibitors from Phlomis tuberosa by Sepbox Chromatography and Thin-Layer Chromatography Bioautography

Alpha-glucosidase inhibitors currently form an important basis for developing novel drugs for diabetes treatment. In our preliminary tests, the ethyl acetate fraction of Phlomis tuberosa extracts showed significant α-glucosidase inhibitory activity (IC₅₀ = 100 μg/mL). In the present study, a combined method using Sepbox chromatography and thin-layer chromatography (TLC) bioautography was develo...

Interactions of Quaternary Ammonium Compounds and Proteins

A simple met.hod of titration with a cationic detergent has been developed which permits rapid estimation of the concentration of urinary protein with a minimum of manipulations and time. The method depends on the formation of an insoluble anion-cation complex between quaternary ammonium ions, hereinafter referred to as the cationic detergent, and proteins, in the present instance mainly human ...

Bile Compounds: Thin-layer (planar) Chromatography

With regard to the liquid chromatographic methods described for bile acid analysis, especially with regard to their determination in biological matrices, several approaches for qualitative and quantitative determination have been described. A critical evaluation of the advantages and disadvantages of these methods results in the conclusion that there are speciRc limitations for every applicatio...