Analysis of K99 plasmids from enterotoxigenicEscherichia coli

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Analysis of a naturally occurring K99+ enterotoxigenic Escherichia coli strain that fails to produce K99.

A spontaneously occurring field isolate of enterotoxigenic Escherichia coli that was genotypically K99+ but phenotypically K99- was analyzed for the reason that it did not express K99. The defect, which was cis active, was located within an area 5' to the first gene required for K99 biogenesis and was the result of the deletion of a single base pair.

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Mosaic structure of plasmids from natural populations of Escherichia coli.

The distribution of plasmids related to the fertility factor F was examined in the ECOR reference collection of Escherichia coli. Probes specific for four F-related genes were isolated and used to survey the collection by DNA hybridization. To estimate the genetic diversity of genes in F-like plasmids, DNA sequences were obtained for four plasmid genes. The phylogenetic relationships among the ...

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Replication of plasmids from Staphylococcus aureus in Escherichia coli.

Plasmid pBR322 derives from plasmid ColE1 and does not replicate in Escherichia coli strains lacking DNA polymerase I. Hybrids between pBR322 and a plasmid isolated from Staphylococcus aureus, pC194, replicate in such E. coli strains, provided that the pC194 replication region is intact. Inactivation of the pBR322 replication region does not interfere with the replication of hybrids in E. coli....

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ژورنال

عنوان ژورنال: FEMS Microbiology Letters

سال: 1992

ISSN: 0378-1097,1574-6968

DOI: 10.1111/j.1574-6968.1992.tb05141.x