Analysis of Heat Flow for In Vitro Culture Monitored by Impedance Measurement

The paper proposes an innovative method of using microheaters for research on cells in vitro. A local heating a single culture well, compatible with Electric Cell-substrate Impedance (ECIS) system is presented. microheater and well cell was modelled. Electrical thermal simulation the under operating conditions carried out. Correct distribution heat observed at site suspension medium, while not affecting adjacent wells. As part experiment, element nichrome (NiCr) created magnetron sputtering process. measurements manufactured device were It has been shown that it possible to establish desired temperature over long term. In addition, structures made characterised by work stability, precision maintaining right temperature, possibility being controlled high accuracy. There problem precise reproducible carrying out experiment differs only process temperature. this work, technique increasing locally, medium containing eight such wells proposed analyzed. use will allow avoidance impact potential changes parameters other than culture. That may occur when comparing grown different temperatures means ECIS (Electric Impedance) method.