Analysis of genetic diversity in Prunus sibirica L. in inner Mongolia using SCoT molecular markers

Abstract Population genetic diversity contributes to the protection and utilization of germplasm resources, especially via breeding. In present study, start codon targeted polymorphism (SCoT) molecular markers were used study 278 individuals from 10 Prunus sibirica L. populations in Inner Mongolia. A total 289 polymorphic bands amplified with 23 SCoT primers, showing a percentage 98.87% an average 12.6 per primer. The SCoT21, SCoT32, SCoT53 primers up 17 bands, was 100%. minimum number by SCoT25 9, 90%. Therefore, shown be highly suitable for studies P. analysis variance showed that 39% observed differentiation occurred among 61% within populations, indicating greater than populations. results unweighted pair-group method arithmetic cluster analysis, principal coordinate STRUCTURE basically same divided into 2 groups. indicated efficient marker-based Mongolia can provide reference variety breeding resource development.