Analysis of codon usage pattern in Lonicera × heckrottii ‘Gold Flame’ based on chloroplast genome

Codon usage bias (CUB) was a unique feature of the genome, and revealing chloroplast genome codon can provide useful information for evolution plant species. Lonicera × heckrottii ‘Gold Flame’ is one members genus with important ornamental value. However, this genotype has not been investigated. In study, base compositions various indices 51 coding sequences from calculated, by W, DnaSP, CUSP EMBOSS SPSS software. A total CDS selected to analyse bias. The results showed that average GC content 39.27%, value ENC 48.75%. gene weak, preferred A/T ending. general order GC1 (47.72%)>GC2 (39.89%)>GC3 (30.19%). Correlation analysis there significant positive correlation between GC1, GC2 GC3 content. Combined neutral plot analysis, ENC-plot PR2-plot it found preference affected mutations pressure natural selection. addition, eight optimal codons in were finally identified, ending A/T. study on provides demonstration exploring its genetic structure molecular mechanism, reference breeding.