Analysis of CcpA mutations defective in carbon catabolite repression in Bacillus megaterium
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چکیده
منابع مشابه
Analysis of CcpA mutations defective in carbon catabolite repression in Bacillus megaterium.
Five mutations in ccpA of Bacillus megaterium with impaired functions were analysed for carbon catabolite repression. The phenotypes support the hypothesis that CcpA assumes a PurR/LacI fold. The completely inactive mutants CcpA119GE and CcpA326am cause alterations which are incompatible with that fold. A mutation with reduced activity, CcpA81GE, affects a site that would be partially surface e...
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Carbon catabolite repression (CCR) by transcriptional regulators follows different mechanisms in gram-positive and gram-negative bacteria. In gram-positive bacteria, CcpA-dependent CCR is mediated by phosphorylation of the phosphoenolpyruvate:sugar phosphotransferase system intermediate HPr at a serine residue at the expense of ATP. The reaction is catalyzed by HPr kinase, which is activated by...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2006
ISSN: 0378-1097
DOI: 10.1111/j.1574-6968.1997.tb10485.x