Anaerobic co-digestion of sewage sludge and Cladophora green algae: Investigation of synergistic effects and Optimization of substrate composition ratio

Anaerobic co-digestion of primary and secondary sewage sludge <i>Cladophora</i> green algae was investigated under mesophilic temperature conditions. The design experiment method the optimal mixture were used to systematically optimize substrate composition ratios elucidate possible synergistic effects for an anaerobic digestion system. A reduced cubic model created by Design-Expert software as a function ratios. validated ANOVA experimentally. Also, ratio's on variations in biogas production studied. All linear impacts changes significantly observed, interactions between substrates combined had rate. highest amount (235.17-296.03 ml/g VS) obtained with equal 70-60%, B 0-17%, C 18-30%. Eventually, optimization performed predict conditions achieve maximum It seen that predicted actual values produced favorable error about 1.3% are well consistent. authors conclude can be utilized assess