An Improved Purification Method for Removing Colour Interference from 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) Antibacterial Assays
نویسندگان
چکیده
The MTT antibacterial assay is an important method in natural component discovery. Researchers can use the to quickly and efficiently evaluate activity of compounds. aim this research was find a reduce background colour bacterial cell interference when using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) study effect phytochemicals. This used NZ ‘Hass’ ripe avocado seed extract as example develop silica gel column chromatography that could combine with remove cells before formazan measuring stage. particularly suitable for MTT-based inhibition studies tested phytochemical extracts have interfering colour.
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ژورنال
عنوان ژورنال: Applied sciences
سال: 2023
ISSN: ['2076-3417']
DOI: https://doi.org/10.3390/app13085067