An improved plasmid for transformation of Neurospora crassa using the pan-2 gene as a selectable marker
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چکیده
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Position-independent germline transformation in Drosophila using a cuticle pigmentation gene as a selectable marker.
P element mediated germline transformation is an essential part of the analysis of gene function in Drosophila melanogaster. This method allows for efficient introduction of single copies of genes into random positions within the genome (1). The generation of multiple transformed lines is required, as some genes display variable expression patterns depending on the insertion site (2, 3). Elimin...
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A mus-51 RIP allele for transformation of Neurospora crassa.
This report describes the construction and characterization of mus-51RIP70 , an allele for high-efficiency targeted integration of transgenes into the genome of the model eukaryote Neurospora crassa. Two of the mus-51RIP70 strains investigated in this work (RZS27.10 and RZS27.18) can be obtained from the Fungal Genetics Stock Center. The two deposited strains are, to our knowledge, genetically ...
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The objective of this research was to establish a chloroplast transformation technique for Platymonas (Tetraselmis) subcordiformis. Employing the gfp gene as a reporter and the bar gene as a selectable marker, transformation vectors of P. subcordiformis chloroplast were constructed with endogenous fragments rrn16S-trnI (left) and trnA-rrn23S (right) as a recombination site of the chloroplast ge...
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 2008
ISSN: 1941-4765
DOI: 10.4148/1941-4765.1091