An improved PCR method for walking in uncloned genomic DNA

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An improved PCR method for walking in uncloned genomic DNA.

Several PCR-based methods are available for walking from a known region to an unknown region in cloned or uncloned genomic DNA. The methods are of three types: inverse PCR (1), randomly primed PCR (2) and adaptor ligation PCR (3-6). However, these methods have not been generally applied to walking in uncloned genomic DNA because they are either complicated or inefficient. Recent improvements to...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1995

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/23.6.1087