An improved method for constructing and selectively silanizing double-barreled, neutral liquid-carrier, ion-selective microelectrodes
نویسندگان
چکیده
منابع مشابه
Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue.
Electrical activity in the brain is accompanied by significant ion fluxes across membranes, resulting in complex changes in the extracellular concentration of all major ions. As these ion shifts bear significant functional consequences, their quantitative determination is often required to understand the function and dysfunction of neural networks under physiological and pathophysiological cond...
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Determining the effective concentration (i.e., activity) of ions in and around living cells is important to our understanding of the contribution of those ions to cellular function. Moreover, monitoring changes in ion activities in and around cells is informative about the actions of the transporters and/or channels operating in the cell membrane. The activity of an ion can be measured using a ...
متن کاملSimultaneous Measurement of Intracellular pH and K+ or NO3- in Barley Root Cells Using Triple-Barreled, Ion-Selective Microelectrodes.
The manufacture and use of triple-barreled microelectrodes, which are capable of simultaneous in vivo measurement of intracellular pH and the activities of K+ or NO3- and cell membrane potential (Em), are described. Scanning electron micrographs showed that the three tips were aligned and that the overall tip diameter was approximately 0.8 [mu]m. When filled with 100 mM KCl, all three barrels s...
متن کاملThe production and testing of double-barreled pH glass microelectrodes for measurement of intratubular pH.
It lhas been possible to miniatuirize the glass pH electrode to an extent tllat it can be uised to measture the intraltiminal pH of single mammalian nephrons(l). There are, however, a nutmber of problems that arise from the miniaturization process. Thlese includ(le electrode insulation, the incorporation of a suitable reference electrode witlh the pH glass electrode, an(d the ultimate achievabl...
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ژورنال
عنوان ژورنال: Biological Procedures Online
سال: 2005
ISSN: 1480-9222
DOI: 10.1251/bpo103