An Image Segmentation Method for Wound Healing Assay Images

Wound healing assays are important for molecular biologists to understand the
 mechanisms of cell migration. For the analysis wound assays, accurate
 segmentation front is a necessity. Manual annotation is
 inconvenient since it time-consuming and annotator-dependent. Thus automated, fast,
 robust solutions required. There several image processing techniques
 proposed fulfill this need. However, requirement specification optimal
 parameters, need human intervention, lack high accuracy emerge as
 downfalls most them. In study we have novel method to
 overcome these difficulties.