An Efficient Agrobacterium ‐Mediated Transformation Protocol for Hexaploid and Tetraploid Wheat
نویسندگان
چکیده
منابع مشابه
Generation of marker‐free transgenic hexaploid wheat via an Agrobacterium‐mediated co‐transformation strategy in commercial Chinese wheat varieties
Genotype specificity is a big problem lagging the development of efficient hexaploid wheat transformation system. Increasingly, the biosecurity of genetically modified organisms is garnering public attention, so the generation of marker-free transgenic plants is very important to the eventual potential commercial release of transgenic wheat. In this study, 15 commercial Chinese hexaploid wheat ...
متن کاملAn Improved Agrobacterium Mediated Transformation System in Wheat
Wheat is the world’s largest volume crop traded internationally. Foliar diseases are the main biotic restriction reducing yield in wheat crops. Wheat productivity can be enhanced through controlling foliar disease through the use of resistant cultivars. Plant breeders are providing considerable attention to wheat genetic improvement from the past few years to minimize losses due to pests and pa...
متن کاملAn Efficient Agrobacterium-Mediated Transformation System for Poplar
Poplar is a model system for the regeneration and genetic transformation of woody plants. To shorten the time required for studies of transgenic poplar, efforts have been made to optimize transformation methods that use Agrobacterium tumefaciens. In this study, an Agrobacterium infective suspension was treated at 4 °C for at least 10 h before infecting explants. By transforming the Populus hybr...
متن کاملAn efficient protocol for peanut (Arachis hypogaea L.) transformation mediated by Agrobacterium rhizogenes
The Agrobacterium-mediated transformation of hairy roots creates a rapid and simple means to introduce and express foreign genes in plant cells that are capable of synthesizing specific natural products. We describe a method for the production of transgenic hairy root cultures of peanut (Arachis hypogaea L.) using Agrobacterium rhizogenes R1000, a strain with the binary vector pBI121. We evalua...
متن کاملEnsifer-mediated transformation: an efficient non-Agrobacterium protocol for the genetic modification of rice
While Agrobacterium-mediated transformation (AMT) remains the most widely used technique for gene transfer in plants, interest exists for the use of non-Agrobacterium gene delivery systems due to freedom-to-operate issues that remain with AMT across several jurisdictions. In addition, the plant pathogenic mode of action of Agrobacterium tumefaciens significantly increases the costs to passage e...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Protocols
سال: 2021
ISSN: 2691-1299,2691-1299
DOI: 10.1002/cpz1.58