An Automated Determination of Alkaline Phosphatase Utilizing p-Nitrophenol Phosphate
نویسندگان
چکیده
منابع مشابه
Phosphate Binding to Alkaline Phosphatase
The metal ion dependence of a2P binding to Escherichia coli alkaline phosphatase has been studied by means of equilibrium dialysis. Whereas the apoenzyme does not bind phosphate (no sites with K < 5 X 10-5 M), the addition of 2 Zn(I1) cations per molecule induces the tight binding of 1 phosphate dianion (K = 6 X 1OF M). The magnitude of this binding constant is affected by ionic strength, pH, a...
متن کاملAn automated p-nitrophenylphosphate serum alkaline phosphatase procedure for the AutoAnalyzer.
A new and simplified procedure for the automated determination of serum alkaline phosphatase uses the AutoAnalyzer. The substrate p-nitrophenylphosphate in 2amino-2-methyl-l-propanol offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. The procedure also permits use of small sample volumes, provides linear reaction rates, and has a simple m...
متن کاملAn automated p-nitrophenylphosphate serum alkaline phosphatase procedure for the "Robot Chemist".
A new, precise, and accurate procedure is described for the automated determination of serum alkaline phosphatase on the Robot Chemist. The substrate, p-nitrophenylphosphate in 2-amino-2-methyl-1-propanol, offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. Other advantages are the small sample volumes required and linear reaction rates. C...
متن کاملAn automated alkaline phosphatase assay with phenolphthalein monophosphate as substrate.
The manual procedure of Babson et al. (1) for the determination of serum alkaline phosphatase has been automated. The assay is based on the colorimetric determination of phenolphthalein formed on hydrolysis of phenolphthalein monophosphate. The procedure utilizes 0.16 ml. of serum without dialysis. Blanks are required only for turbid sera. Results are compared with those obtained by the manual ...
متن کاملAutomated determination of acid phosphatase.
A procedureis presentedfor the automated determination of acid phosphatase activity in biological materials from either plant or animal origin. The Technicon N-i flow diagram has been simplified, and the reagents for the measurement of enzymatically produced phenolic substanceshave been modified without lossof range or sensitivity. Both phenyiphosphateand a-naphthylphosphate, introduced by Babs...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1964
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/10.12.1112