An Assay for the Receptor-Destroying Activity of Influenza C Virus
نویسندگان
چکیده
منابع مشابه
A Sensitive Neutralization Assay for Influenza C Viruses Based on the Acetylesterase Activity HEF Glycoprotein
Influenza C virus possesses specific neuraminate-O-acetylesterase as a receptor-destroying function. This enzymatic activity of the viral glycoprotein HEF (Hemagglutinin, esterase activity and fusion factor) can be visualized in situ by the use of distinct color substrates. Hereby the localization, as well as the quantity of synthesized HEF protein is detectable. We further developed the estera...
متن کاملa sensitive neutralization assay for influenza c viruses based on the acetylesterase activity hef glycoprotein
influenza c virus possesses specific neuraminate-o-acetylesterase as a receptor-destroying function. this enzymatic activity of the viral glycoprotein hef (hemagglutinin, esterase activity and fusion factor) can be visualized in situ by the use of distinct color substrates. hereby the localization, as well as the quantity of synthesized hef protein is detectable. we further developed the estera...
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The direct F.A. Method has been used for differentiation of virulent and avirulent lines of influenza viruses. A positive fluorescence was obtained indicating the level of virus in the tissues, sorbing with dried mouse powder helped to reduce non-specific staining. The virulent line showed the greatest number of fluorescent cells with the peak at 10 hours. the non-virulent lines showed a gra...
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Infectious salmon anaemia virus (ISAV) infects cells via the endocytic pathway and, like many other enveloped viruses, ISAV contains a receptor-destroying enzyme. We have analysed this acetylesterase activity with respect to substrate specificity, enzyme kinetics, inhibitors, temperature and pH stability. The ISAV acetylesterase was inhibited by di-isopropyl fluorophosphate (DFP) in a dose-depe...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 1985
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.1985.tb00910.x