An Artificial Compartmentalized Biocatalytic Cascade System Constructed With Enzyme‐Caged Reticulate Nanoporous Membranes

Compartmentalization is a ubiquitous feature of life, with membrane interface that regulates molecular transport and exhibits bioactivities. An artificial enzyme-integrated cascade system can mimic such interactive properties across different length scales for in vitro application. Here, it shown reticulated nanoporous framework nano-caged enzymes presents the first modular macroscale platform compartmentalized biocatalytic interfaced an external medium. Catalase (CAT)-integrated polyurea scaffold prepared by simple pressurization procedure model cyclic glucose oxidase/catalase (GOx/CAT). The bicontinuous readily constructs interfacing glucose/GOx solution environment mediates oxidation reaction under anaerobic or aerobic conditions. Furthermore, compartment multiple bioactive capabilities barrier properties, including hydrogen peroxide detoxification, situ oxygen generation, protease exclusion. This work suggests new strategy toward robust membranous to mediate biochemical cascades occurring environment, promising potential applications biohybrid devices embedded tissues cells.