An aroA mutant of Yersinia pestis is attenuated in guinea-pigs, but virulent in mice
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منابع مشابه
An aroA mutant of Yersinia pestis is attenuated in guinea-pigs, but virulent in mice.
This study describes a PCR-based approach for the production of a rationally attenuated mutant of Yersinia pestis. Degenerate primers were used to amplify a fragment encoding 91.45% of the aroA gene of Y. pestis MP6 which was cloned into pUC18. The remainder of the gene was isolated by inverse PCR. The gene was sequenced and a restriction map was generated. The Y. pestis aroA gene had 75.9% ide...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1996
ISSN: 1350-0872,1465-2080
DOI: 10.1099/13500872-142-7-1847