An alternative strategy for cloning minor permeases in Aspergillus nidulans

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Aspergillus nidulans

Light-dependent conidiation in the filamentous ascomycete, Aspergillus nidulans, is contingent on the allelic state of the velvet (veA) gene. Light dependence is abolished by a mutation in this gene (veAl) , which allows conidiation to occur in the absence of light. We have isolated and characterized six extragenic suppressors of veAl that restore the light-dependent conidiation phenotype. Alle...

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Mitosis in Aspergillus nidulans.

Mitosis in growing hyphae of haploid and diploid Aspergillus nidulans has been studied with the light microscope in Helly-fixed preparations. At the periphery of the nucleus a sharply defined granule is invariably found which has strong affinity for acid fuchsin. Mitosis begins with the duplication of this granule and the development inside the nucleus of a gradually lengthening fibre between t...

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Cloning and characterization of an Aspergillus nidulans gene involved in the regulation of penicillin biosynthesis.

To identify regulators of penicillin biosynthesis, a previously isolated mutant of Aspergillus nidulans (Prg-1) which carried the trans-acting prgA1 mutation was used. This mutant also contained fusions of the penicillin biosynthesis genes acvA and ipnA with reporter genes (acvA-uidA and ipnA-lacZ) integrated in a double-copy arrangement at the chromosomal argB gene. The prgA1 mutant strain exh...

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Transformation of Aspergillus nidulans

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ژورنال

عنوان ژورنال: Fungal Genetics Reports

سال: 2000

ISSN: 1941-4765

DOI: 10.4148/1941-4765.1203