An Allochrome Periodic Acid-Methenamine Silver Stain for Demonstrating Mycobacteria in Tissue Sections

An allochrome periodic acid-methenamine silver stain for demonstrating mycobacteria in tissue sections.

Nyka9) employed the Gomori-Grocott's chromic acid-methenamine silver method for demonstrating tubercle bacilli in tissue sections after sublimate fixative with added hydrogen peroxide or periodic acid. He described that this procedure could demonstrate mycobacteria that had lost their acidfastness by the usual carbol fuchsin stain. Harada5) also employed the periodic acid-methenamine silver sta...

The use of non-deparaffinized tissue sections for staining leprosy bacilli.

Reduced acid-fast staining of leprosy bacilli occurs during the dewaxing of paraffin sections by xylene and alcohols; the older and more decrepit bacilli being especially affected. By the use of non-deparaffinized sections, the leprosy bacilli which could not be stained with the usual carbol fuchsin are strongly stained. Moreover, non-deparafinized sections can be used for the periodic acid-car...

Direct immunoperoxidase method for demonstrating Chlamydia psittaci in tissue sections.

Orcein as a mucin stain for gastrointestinal tissue sections.

Although orcein has been in use for a long time as a stain for elastic fibres, it is only recently that it has been rediscovered for the demonstration of a variety of other substances. These include hepatitis B surface antigen (HBsAg),' 2 copper-associated protein,34 and more recently, intestinal sulphated mucins.35 Sipponen5 studied the normal distribution of orceinpositive mucin in the gastro...

The periodic acid-Schiff stain for the demonstration of fungi in animal tissue.

The routine histopathologic stains used for the demonstration of fungi in animal tissue are inadequate. The fungi are differentiated poorly and sometimes not at all. This is a limitation in the diagnosis of the mycotic granulomas in which the organisms may be rare. A definitive fungus stain, therefore, is needed, particularly by the pathologist and mycologist. During an attempt to stain the cap...