Amplifiable Carbohydrate Mimicries Provided by Peptide-Displaying Phage
نویسندگان
چکیده
منابع مشابه
Peptide-displaying phage technology in glycobiology.
Phage display technology is an emerging drug discovery tool. Using that approach, short peptides that mimic part of a carbohydrate's conformation are selected by screening a peptide-displaying phage library with anti-carbohydrate antibodies. Chemically synthesized peptides with an identified sequence have been used as an alternative ligand to carbohydrate-binding proteins. These peptides repres...
متن کاملTriple tandem mimotope peptide of Epidermal Growth Factor Receptor displaying on the surface of M13 phage induces anti-tumor response in mice tumor model
Introduction: Epidermal growth factor receptor (EGFR) has been shown to play a critical role in tumor cell growth and its overexpression has been observed in many epithelial tumors. In the field of cancer vaccine research, displaying the peptide mimotope on the surface of phage particles has shown promising results. Methods: In this study using m13-PVIII phage display system, two constructs we...
متن کاملAnti-tumor immunity provided by a synthetic multiple antigenic glycopeptide displaying a tri-Tn glycotope.
In many cancer cells the alteration of glycosylation processes leads to the expression of cryptic carbohydrate moieties, which make them good targets for immune intervention. Identification of cancer-associated glycotopes as well as progress in chemical synthesis have opened up the way for the development of fully synthetic immunogens that can induce anti-saccharide immune responses. Here, we s...
متن کاملDirect immunoprecipitation of antigen with phage displaying immunoglobulin fragment.
Phage libraries may display hormones, receptors, antibody fragments, etc., by fusion to phage envelope proteins. This report describes the direct precipitation of phage-Fab-antigen complexes by polyethylene glycol precipitation, resulting in highly selective and efficient recovery of antigen from complex mixtures without nonspecific protein contamination. The method demonstrates efficiency and ...
متن کاملSoftware Tools for Storing, Processing and Displaying Carbohydrate Microarray Data
We describe a suite of software modules to store, retrieve and display carbohydrate microarray data. Storage is in a relational database that holds all the microarray data and associated glycan, protein and experimental information. The retrieval and display software has a comprehensive system of sorters, filters and arrangers to allow highly customized presentation of data as charts, tables, 2...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Trends in Glycoscience and Glycotechnology
سال: 2006
ISSN: 0915-7352,1883-2113
DOI: 10.4052/tigg.18.147