Amperometric Titration of Sulfhydryl and Disulfide Groups in Milk Proteins
نویسندگان
چکیده
منابع مشابه
Amperometric titration of disulfide and sulfhydryl in proteins in 8 M urea.
Of the numerous methods which have been used in the estimation of disulfide bonds and sulfhydryl groups in proteins and amino acids (l), the amperometric, argentimetric titration procedure, with appropriate modifications, is especially well suited for this purpose. This paper describes a modification and extension of known methods whereby these groups may be titrated in 8 M urea. The total numb...
متن کاملSulfhydryl and Disulfide Groups of Proteins
1. Methods have been described for reducing protein S-S groups, for oxidizing protein SH groups, and for estimating protein S-S and SH groups. 2. It has been found necessary in estimating the cystine content of proteins by the Folin-Marenzi method to take into account any cysteine that may be present. 3. A method for estimating the cysteine content of proteins has been described. 4. With these ...
متن کاملSulfhydryl and Disulfide Groups of Proteins Iv. Sulfhydryl Groups of the Proteins of ~uscle by A. E. Mirsky
By investigating the sulfhydryl groups of the muscle proteins I have attempted to throw some light on the state of the proteins in muscle and on the change in state of these proteins in muscular contraction. In previous papers i t has been shown that in a denatured protein the number of active SH groups is equivalent to the quantity of cysteine found in the hydrolyzed protein (Mirsky and Anson,...
متن کاملSulfhydryl and Disulfide Groups of Proteins Iv. Sulfhydryl Groups of the Proteins of ~uscle by A. E. Mirsky
By investigating the sulfhydryl groups of the muscle proteins I have attempted to throw some light on the state of the proteins in muscle and on the change in state of these proteins in muscular contraction. In previous papers i t has been shown that in a denatured protein the number of active SH groups is equivalent to the quantity of cysteine found in the hydrolyzed protein (Mirsky and Anson,...
متن کاملSulfhydryl Groups in Proteins
Egg albumin in the native, unaltered state, does not give tests characteristic of sulfhydryl groups. When, however, this protein is treated in any one of several ways, such as by heat (8, 9, 17), ultraviolet irradiation (lo), shaking (lo), or by solution in urea or other amides (7), free sulfhydryl groups make their appearance. The amount of free -SH groups appearing in egg albumin through the ...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1962
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(62)89656-8