Amino acid sequences of proteins from Leptospira serovar pomona
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چکیده
منابع مشابه
Amino acid sequences of proteins from Leptospira serovar pomona.
This report describes a partial amino acid sequences from three putative outer envelope proteins from Leptospira serovar pomona. In order to obtain internal fragments for protein sequencing, enzymatic and chemical digestion was performed. The enzyme clostripain was used to digest the proteins 32 and 45 kDa. In situ digestion of 40 kDa molecular weight protein was accomplished using cyanogen bro...
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Leptospira interrogans is a diverse species in which individual serovars have distinctive restriction fragment length polymorphisms that are useful in strain identification. Many of these polymorphisms can be detected using hybridization probes derived from insertion sequences; an observation that suggests these IS elements are active and can transpose in L. interrogans. Two spontaneous mutants...
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A plate assay which utilizes the addition of a blood agar overlay for detection of hemolysin by colonies of Leptospira interrogans serovar pomona is described.
متن کاملCopurification of Leptospira interrogans serovar pomona hemolysin and sphingomyelinase C.
The hemolytic and sphingomyelinase C activities of supernatants of cultures of Leptospira interrogans serovar pomona tended to copurify when isoelectric fractionation was carried out. Both activities focused primarily at pH 8.1. Considered in conjunction with other circumstantial evidence, the results led to the conclusion that sphingomyelinase C is responsible for hemolysis.
متن کاملDistinguishing Proteins From Arbitrary Amino Acid Sequences
What kinds of amino acid sequences could possibly be protein sequences? From all existing databases that we can find, known proteins are only a small fraction of all possible combinations of amino acids. Beginning with Sanger's first detailed determination of a protein sequence in 1952, previous studies have focused on describing the structure of existing protein sequences in order to construct...
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ژورنال
عنوان ژورنال: Memórias do Instituto Oswaldo Cruz
سال: 2000
ISSN: 0074-0276
DOI: 10.1590/s0074-02762000000400010