Amino acid sequence of ovine 6-phosphogluconate dehydrogenase.
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چکیده
منابع مشابه
Geobacillus stearothermophilus 6-phosphogluconate dehydrogenase complexed with 6-phosphogluconate
Two crystal structures of recombinant Geobacillus stearothermophilus 6-phosphogluconate dehydrogenase (Gs6PDH) in complex with the substrate 6-phosphogluconate have been determined at medium resolution. Gs6PDH shares significant sequence identity and structural similarity with the enzymes from Lactococcus lactis, sheep liver and the protozoan parasite Trypanosoma brucei, for which a range of st...
متن کاملDietary regulation of 6-phosphogluconate dehydrogenase synthesis.
The relative rates of synthesis and degradation for rat liver 6-phosphogluconate dehydrogenase have been determined in animals maintained at several dietary states. Relative rates of synthesis were determined by pulse-labeling the enzyme either in live rats and determining the radioactivity in the purified enzyme or in whole cell suspensions of hepatocytes followed by precipitation of the enzym...
متن کامل6-Phosphogluconate dehydrogenase from leuconostoc mesenteroides.
The pathways for degradation of 6-phosphogluconate have been rather clearly defined for several organisms, the most notable of which are yeast (Horecker, 1953), Escherichia coli (Cohen, 1951), Pseudomonas saccharophila (Entner and Doudoroff, 1952; MacGee and Doudoroff, 1954), and Pseudomonas fluorescens (Kovachevich and Wood, 1954). The enzymes from yeast and E. coli appear to be similar, if no...
متن کاملThe Mechanism of Action of 6-phosphogluconate Dehydrogenase.
6-Phosphogluconate dehydrogenase from Candida utilis catalyzes the oxidative decarboxylation of 2-deoxy-6-phosphogluconate. The 3-keto-2-deoxy-6-phosphogluconate, an intermediate of the reaction, is reduced to 2-deoxy-6-phosphogluconate and decarboxylated to I-deoxyribulose 5-phosphate when incubated with the enzyme and TPNH. The decarboxylation process does not occur in the absence of the redu...
متن کاملMicrofluorometry of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in red cells.
A simple microfluorometric method isdescribed for measu ring gIucose-6-phosphate dehyd rogenase (GPD) and 6-phosphogluconate dehydrogenase (PGD) activities in human erythrocytes. Only 50 ,J of whole blood is required. The cells are lysed with digitonin and the rate of NADPH generated in the enzymatic reactions is followed kinetically with a recording fluoromete. GPD activity is measured by subt...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1983
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)44055-5