Amethod of Evaluation False-positive EGFR T790M Mutation Based on Deamining 5-methylcytosine

The epidermal growth factor receptor (EGFR) T790M mutation, a missense mutation from CpG cytosine to thymine, in non-small cell lung cancer is drug-resistant induced by the administration of tyrosine kinase inhibitors (TKIs). It important detect this with high sensitivity, as effective therapies have been developed for patients mutation. We confirmed that at point methylated various tissues, and 5-methylcytosine readily deaminated hydrolysis converted which detected an artifact. new method estimate artifact formalin fixed paraffin-embedded (FFPE) specimens evaluating mutant ratio other 5-methylcytosine, enabling sensitive accurate detection