Amelogenin-Ameloblastin Spatial Interaction around Maturing Enamel Rods
نویسندگان
چکیده
منابع مشابه
Tracking Endogenous Amelogenin and Ameloblastin In Vivo
Research on enamel matrix proteins (EMPs) is centered on understanding their role in enamel biomineralization and their bioactivity for tissue engineering. While therapeutic application of EMPs has been widely documented, their expression and biological function in non-enamel tissues is unclear. Our first aim was to screen for amelogenin (AMELX) and ameloblastin (AMBN) gene expression in mandib...
متن کاملAmelogenin-cytokeratin 14 interaction in ameloblasts during enamel formation.
The enamel protein amelogenin binds to the GlcNAc-mimicking peptide (GMp) (Ravindranath, R. M. H., Tam, W., Nguyen, P., and Fincham, A. G. (2000) J. Biol. Chem. 275, 39654-39661). The GMp motif is found in the N-terminal region of CK14, a differentiation marker for ameloblasts. The binding affinity of CK14 and amelogenin was confirmed by dosimetric binding of CK14 to recombinant amelogenin (rM1...
متن کاملAmeloblastin peptide encoded by exon 5 interacts with amelogenin N-terminus
Interactions between enamel matrix proteins are important for enamel biomineralization. In recent in situ studies, we showed that the N-terminal proteolytic product of ameloblastin co-localized with amelogenin around the prism boundaries. However, the molecular mechanisms of such interactions are still unclear. Here, in order to determine the interacting domains between amelogenin and ameloblas...
متن کاملNovel biological activity of ameloblastin in enamel matrix derivative
OBJECTIVE Enamel matrix derivative (EMD) is used clinically to promote periodontal tissue regeneration. However, the effects of EMD on gingival epithelial cells during regeneration of periodontal tissues are unclear. In this in vitro study, we purified ameloblastin from EMD and investigated its biological effects on epithelial cells. MATERIAL AND METHODS Bioactive fractions were purified from...
متن کاملAnalysis of co-assembly and co-localization of ameloblastin and amelogenin
Epithelially-derived ameloblasts secrete extracellular matrix proteins including amelogenin, enamelin, and ameloblastin. Complex intermolecular interactions among these proteins are believed to be important in controlling enamel formation. Here we provide in vitro and in vivo evidence of co-assembly and co-localization of ameloblastin with amelogenin using both biophysical and immunohistochemic...
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ژورنال
عنوان ژورنال: Journal of Dental Research
سال: 2016
ISSN: 0022-0345,1544-0591
DOI: 10.1177/0022034516645389