منابع مشابه
Progress in low-resolution ab initio phasing with CrowdPhase
Ab initio phasing by direct computational methods in low-resolution X-ray crystallography is a long-standing challenge. A common approach is to consider it as two subproblems: sampling of phase space and identification of the correct solution. While the former is amenable to a myriad of search algorithms, devising a reliable target function for the latter problem remains an open question. Here,...
متن کاملAb initio molecular-replacement phasing for symmetric helical membrane proteins
Obtaining phases for X-ray diffraction data can be a rate-limiting step in structure determination. Taking advantage of constraints specific to membrane proteins, an ab initio molecular-replacement method has been developed for phasing X-ray diffraction data for symmetric helical membrane proteins without prior knowledge of their structure or heavy-atom derivatives. The described method is base...
متن کاملAb initio phasing of 3-dimensional partially coherent diffraction patterns
Ever since Thomas Young demonstrated interference effects of light with his famous doubleslit experiment, there has been intense interest in understanding its wave nature. An intense area of research has focussed on determining the interference properties of the light wavefield via its coherence properties, which play an important role in imaging applications. One imaging technique that has exp...
متن کاملAb Initio Phasing by Dual-Space Direct Methods
Shake-and-Bake is an ab initio direct method for solving the crystallographic phase problem. Its most distinctive feature is the repeated alternation of reciprocal-space phase refinement with a complementary real-space process that seeks to improve phases by applying constraints. The Shake-and-Bake philosophy has been implemented in two independent computer programs, SnB and SHELXD. These progr...
متن کاملVariation of solvent density and low-resolution ab initio phasing
The bulk solvent plays a key role in the direct phasing at low resolution. The connectivitybased phasing method has been tested for the crystal of the tRNA-aspartyl-tRNA synthetase complex. The neutron diffraction data have been measured for different solvent contrast masking different components of the crystal. The obtained low resolution images are perfectly correlated with the solvent level.
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ژورنال
عنوان ژورنال: Science
سال: 1993
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.259.5102.1771