Alterations of RNA splicing patterns in esophagus squamous cell carcinoma

Abstract Alternative splicing (AS) is an important biological process for regulating the expression of various isoforms from a single gene and thus to promote proteome diversity. In this study, RNA-seq data 15 pairs matched esophageal squamous cell carcinoma (ESCC) normal tissue samples as well two lines were analyzed. AS events with significant differences identified between ESCC tissues, which re-annotated find protein coding genes or non-coding RNAs. A total 45,439 found. Of these, 6019 (13.25%) differentially identified. Exon skipping (SE) occupied largest proportion abnormal events. Fifteen differential same trends ΔΨ values in Four pathways 20 processes related pro-metastasis junction migration significantly enriched spliced genes. The upregulated factor SF3B4, regulates 92 events, could be potential prognostic ESCC. Differentially genes, including HNRNPC, VCL, ZNF207, KIAA1217, TPM1 CALD1 are shown sashimi plot. These results suggest that junction- migration-related influenced by abnormalities, aberrant can affected changes. involved SF3B4 was found survival-related presumed regulate multiple cancers. summary, we expressed may development