Alpha Lipoic Acid (ALA) Alleviates Hepatocytes Toxicity of Titanium Dioxide Nanoparticles in Rats

BACKGROUND: Titanium dioxide nanoparticles (TiO2 NPs) uptake may primarily cause adverse effects by inducing oxidative stress, resulting in cell damage, genotoxicity, inflammation, and immune response. To date, there are limited studies investigating the effect of TiO2 NPs on liver health no found a naturally occurring compound able to ameliorate such effect. Thus, this study investigated alpha lipoic acid (ALA) potential for reversing biochemical histopathological changes that exposure causes rat liver.METHODS: Thirty adult male albino rats were divided into: control received distilled water, treated with 50 mg/kg ALA, intoxicated NPs, NPs-intoxicated ALA. Rats sacrificed before blood samples collected assess function using parameters alanine aminotransferase (ALT), aspartate (AST), total bilirubin, albumin protein. Liver tissue homogenates prepared hepatic antioxidant stress superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), malondialdehyde (MDA). sections used analysis caspase-3 immunohistochemical analysis.RESULTS: produced deleterious tissue, as confirmed through results, immunohistochemistry, histological alterations. intoxication induced hepatocyte vacuolation, vessels congestion, biliary proliferation, apoptosis, fibrosis. However, ALA treatment significantly alleviated impact liver.CONCLUSION: Administration 600 resulted degenerative lesions, depletion GSH, apoptosis. these mitigated administration. Therefore, offers protection against intoxication.KEYWORDS: titanium nanoparticles, hepatotoxicity, alpha-lipoic (ALA),