Allostery in p38–docking peptide interaction – an NMR study

NMR Methods to Study Dynamic Allostery

Nuclear magnetic resonance (NMR) spectroscopy provides a unique toolbox of experimental probes for studying dynamic processes on a wide range of timescales, ranging from picoseconds to milliseconds and beyond. Along with NMR hardware developments, recent methodological advancements have enabled the characterization of allosteric proteins at unprecedented detail, revealing intriguing aspects of ...

cAMP-dependent allostery and dynamics in Epac: an NMR view.

Epac (exchange protein directly activated by cAMP) is a critical cAMP receptor, which senses cAMP and couples the cAMP signal to the catalysis of guanine exchange in the Rap substrate. In the present paper, we review the NMR studies that we have undertaken on the CBD (cyclic-nucleotide-binding domain) of Epac1. Our NMR investigations have shown that cAMP controls distal autoinhibitory interacti...

an exploratory study of differential item functioning (dif) in efl reading comprehension

بررسی دلایل عملکرد متفاوت سوالات آزمون درک مطلب به زبان خارجی تاریخچه ی تحقیق درباره ی منابع عملکرد مختلف سوالات (دیف) در آزمون درک مطلب پر است از مجموعه ای از متغیرهای فرضیه ای که چند مورد از مهمترین آنها عبارتند از: جنسیت، آشنایی با موضوع متن، علاقه به موضوع یا محتوای متن، حدس زدن، و عوامل بافت اجتماعی (پی 2004؛ زومبو و گلین 2005). مطالعه حاضر با استناد به فلسفه ی انکارپذیری پوپر عوامل ذکر...

Mapping allostery through the covariance analysis of NMR chemical shifts.

Allostery is a fundamental mechanism of regulation in biology. The residues at the end points of long-range allosteric perturbations are commonly identified by the comparative analyses of structures and dynamics in apo and effector-bound states. However, the networks of interactions mediating the propagation of allosteric signals between the end points often remain elusive. Here we show that th...

Intrinsic disorder and allostery in an ensemble