Allosteric enzymes as biosensors for molecular diagnosis

Allosteric Regulatory Enzymes

Searching for new allosteric sites in enzymes.

The discovery of new allosteric sites generates opportunities for the identification of novel pharmaceuticals and increases our understanding of basic biological processes. Increasingly, allosteric sites are being discovered in various families of proteins by several methods, paving the way for the development of entirely new classes of drugs with a wide range of chemotypes. New allosteric site...

Statistical Mechanics of Allosteric Enzymes.

The concept of allostery in which macromolecules switch between two different conformations is a central theme in biological processes ranging from gene regulation to cell signaling to enzymology. Allosteric enzymes pervade metabolic processes, yet a simple and unified treatment of the effects of allostery in enzymes has been lacking. In this work, we take a step toward this goal by modeling al...

Binding Leverage as a Molecular Basis for Allosteric Regulation

Allosteric regulation involves conformational transitions or fluctuations between a few closely related states, caused by the binding of effector molecules. We introduce a quantity called binding leverage that measures the ability of a binding site to couple to the intrinsic motions of a protein. We use Monte Carlo simulations to generate potential binding sites and either normal modes or pairs...

Biosensors: Way of Diagnosis

Biosensors have revolutionized the way of diagnosis by allowing detection of diseases at earlier stage & detection of altered amount of biomarkers in the body. All biosensors usually involve minimal sample preparation as the biological sensing component is highly selective for the analyte concerned. There are numerous biosensors available now-a-days such as electrochemical biosensors, optical b...