Allogeneic stimulation of cytotoxic T cells by supported planar membranes.

Allogeneic stimulation of cytotoxic T cells by supported planar membranes.

Phospholipid vesicles containing the transmembrane protein H-2Kk spontaneously fuse to form planar membranes when incubated on treated glass surfaces. Pattern photobleaching of fluorescent lipid probes indicates that these planar membranes are continuous and that the lipids are as mobile as they are in conventional fluid bilayers or monolayers. H-2Kk molecules in these planar membranes are immo...

Helper T cells are required for the polyclonal stimulation of cytotoxic T cells by concanavalin A

Concanavalin A stimulation of T-cell cytotoxicity has been shown to be absolutely dependent on helper T-cell collaboration. Thymocytes stimulated with ConA do not differentiate to yield cytotoxic effector cells. However, thymocytes cocultured with irradiated spleen cells as helpers and ConA yield high levels of cytotoxicity. The helper cell bears theta antigens on its surface, is not an adheren...

Accessory molecules utilized by endothelial cells for allogeneic stimulation of T-lymphocytes.

Phagocytosis and allogeneic T cell stimulation by cultured human osteoblast-like cells.

BACKGROUND/AIMS The antigen phenotype of human osteoblast-like cells suggests that they are related to other cellular populations and may also have immunologic functions in common. METHODS Flow cytometry and transmission electron microscopy were used to show the phagocytotic activity of osteoblast-like cells in culture. The allogeneic stimulation of T cells by human osteoblast-like cells was ...

Analysis of cytotoxic T cell responses to intracameral allogeneic tumors.

Injection of P815 mastocytoma tumor cells into the anterior chamber (AC) of BALB/c mice results in progressively growing intraocular tumors, whereas injection of the same dose of tumor cells subcutaneously results in rapid rejection of the tumor. Previous results indicated that AC P815 inoculation results in an unorthodox immune response characterized by suppressed delayed hypersensitivity, ele...