All-electrical antibiotic susceptibility testing within 30 min using silicon nano transistors

Rapid and reliable antibiotic susceptibility testing (AST) platform is highly desired to select the right antibiotics treat infectious disease at early stage. Here, we demonstrate rapid ASTs using nanoscale silicon ion-selective field-effect transistor sensors. Our sensors profile bacterial metabolic kinetics by monitoring metabolism induced acidification in growth media with absence presence of different antibiotics. AST results could be determined from profiles a total assay time less than 30 min for strains. In addition, also distinguish bactericidal mechanisms modes actions. Furthermore, initial concentration an unknown sample, key parameter determine its clinic relevance, estimated based on profiles. demonstrated method all-electrical, label-free technology compatible, holds great promise development high-throughput low-cost point-of-care device.