Alkali-labile structures linked to the 5′ ends ofBacillus subtilisshort DNA chains

Alkali-labile structures linked to the 5' ends of Bacillus subtilis short DNA chains

Alkali-labile portion covalently linked to the 5' ends of Bacillus subtilis short DNA chains, the putative primer RNA for discontinuous DNA synthesis, was isolated and analyzed using a temperature sensitive DNA polymerase I mutant, which accumulates nascent DNA fragments at a restrictive temperature. A novel oligonucleotide structure as well as mono- to triribonucleotide stretches were isolated...

Preferential DNA repair of alkali-labile sites within the active insulin gene.

DNA damage and repair were studied in a DNA fragment containing the insulin gene after treatment of cells with methylnitrosourea. For these studies, two clonal isolates from the same rat insulinoma cell line which differ in that the insulin gene is transcribed in one (RINr 38) and is silent in the other (RINr B2) were utilized. Both the determination of immunologically reactive insulin released...

Identification of DNA Markers Linked to Blooming Time in Almond

In this study flowering time and other important morphologic traits were evaluated during two years in F1 almond progenies of seventy two seedlings from cross between the intermediate flowering Italian cultivar ‘Tuono’ and the extra-late blooming Iranian cultivar ‘Shahrood-12’. A modified-bulk segregant analysis in combination with the application of the 140 RAPD primers, 87 nuclear ...

Application of Boundary Element Method to 3 D Submerged Structures With Open Ends (RESEARCH NOTE)

This paper presents a three dimensional application of direct Boundary-Element Method (BEM) for computing interaction of sinusoidal waves with a large submerged open bottom structure near the floor with finite depth. The wave diffraction problem is formulated within the framework of linearized potential theory and solved numerically with direct BEM. A computer program based on BEM is developed ...

Dna Ends: Just the Beginning