منابع مشابه
ALIX Is Recruited Temporarily into HIV-1 Budding Sites at the End of Gag Assembly
Polymerization of Gag on the inner leaflet of the plasma membrane drives the assembly of Human Immunodeficiency Virus 1 (HIV-1). Gag recruits components of the endosomal sorting complexes required for transport (ESCRT) to facilitate membrane fission and virion release. ESCRT assembly is initiated by recruitment of ALIX and TSG101/ESCRT-I, which bind directly to the viral Gag protein and then re...
متن کاملRequirements for nucleocapsid-mediated regulation of reverse transcription during the late steps of HIV-1 assembly
HIV-1 is a retrovirus replicating within cells by reverse transcribing its genomic RNA (gRNA) into DNA. Within cells, virus assembly requires the structural Gag proteins with few accessory proteins, notably the viral infectivity factor (Vif) and two copies of gRNA as well as cellular factors to converge to the plasma membrane. In this process, the nucleocapsid (NC) domain of Gag binds to the pa...
متن کاملVisualizing HIV-1 assembly.
The assembly of an HIV-1 particle is a complex, multistep process involving several viral and cellular proteins, RNAs and lipids. While many macroscopic and fixed-cell microscopic techniques have provided important insights into the structure of HIV-1 particles and the mechanisms by which they assemble, analysis of individual particles and their assembly in living cells offers the potential of ...
متن کاملSNAREs in HIV-1 assembly
Viruses have a limited number of genes but a complex life cycle and have evolved to utilize numerous host factors to complete their replication. The assembly and budding process of enveloped viruses utilizes numerous cellular factors to facilitate transport from one membrane bound compartment to the other. The host SNARE proteins are widely involved in late stages of vesicular mediated transpor...
متن کاملDifferential requirements for Alix and ESCRT-III in cytokinesis and HIV-1 release.
The ESCRT machinery functions in topologically equivalent membrane fission events, namely multivesicular body formation, the terminal stages of cytokinesis and HIV-1 release. Here, we show that the ESCRT-III-binding protein Alix is recruited to the midbody of dividing cells through binding Cep55 via an evolutionarily conserved peptide. Disruption of Cep55/Alix/ESCRT-III interactions causes form...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2014
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2013.11.424