Alanyl-tRNA synthetase from Escherichia coli, Bombyx mori and Ratus ratus. Existence of common structural features
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چکیده
منابع مشابه
Glycyl- and Alanyl-tRNA Synthetases from Bombyx mori
Glycyland alanyl-tRNA synthetases have been purified from an extract of Bombyx mori posterior silk glands by a procedure that allows for the simultaneous isolation of both enzymes. Glycyl-tRNA synthetase is a dimer of M, = 160,000 consisting of similar or identical subunits, whereas alanyl-tRNA synthetase is a monomer of M, = 110,000 to 116,000. The abundance of these two enzymes in the posteri...
متن کاملPrimary structure of alanyl-tRNA synthetase and the regulation of its mRNA levels in Bombyx mori.
cDNA clones encoding Bombyx mori alanyl-tRNA synthetase were isolated from a library in lambda gt11 using antibody, synthetic oligonucleotides, and a characterized cDNA as probes. Analysis of the sequence revealed significant homology between the B. mori and Escherichia coli alanyl-tRNA synthetases, particularly in their amino-terminal domains. Northern blot analysis indicated that the mRNA for...
متن کاملClone and functional analysis of Seryl-tRNA synthetase and Tyrosyl-tRNA synthetase from silkworm, Bombyx mori
Aminoacyl-tRNA synthetases are the key enzymes for protein synthesis. Glycine, alanine, serine and tyrosine are the major amino acids composing fibroin of silkworm. Among them, the genes of alanyl-tRNA synthetase (AlaRS) and glycyl-tRNA synthetase (GlyRS) have been cloned. In this study, the seryl-tRNA synthetase (SerRS) and tyrosyl-tRNA synthetase (TyrRS) genes from silkworm were cloned. Their...
متن کاملMapping contacts between Escherichia coli alanyl tRNA synthetase and 2' hydroxyls using a complete tRNA molecule.
A dual-specific derivative of yeast tRNA(Phe) is described whose features facilitate structure-function studies of tRNAs. This tRNA has been made in three different bimolecular forms that allow modifications to be easily introduced into any position within the molecule. A set of deoxynucleotide substituted versions of this tRNA has been created and used to examine contacts between tRNA and Esch...
متن کاملProbing the principles of amino acid selection using the alanyl-tRNA synthetase from Escherichia coli.
The alanyl-tRNA synthetase from Escherichia coli activates cysteine, alpha-aminobutyrate and other-noncognate amino acids that are larger than alanine so slowly that no editing mechanism is required for error correction. Serine, however, is activated sufficiently rapidly that an editing mechanism is required to remove the products of misactivation. The distinction between the nominally isosteri...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1991
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1991.tb16002.x