Agrobacterium-produced and exogenous cytokinin-modulated Agrobacterium-mediated plant transformation
نویسندگان
چکیده
منابع مشابه
Soybean: Plant Manipulation to Agrobacterium Mediated Transformation
Revolution in plant biotechnology can be categorized into in vitro culture and genetic transformation. Plant regeneration was successfully achieved in 1950’s while production of transgenic plant was accomplished in 80’s. For production of transformants, in vitro culturing strategies are prerequisite. Soybean has been cultured through organogenesis and embryogenesis but still it is considered re...
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Agrobacterium tumefaciens is a Gram-negative soil bacterium that causes plant tumours by transferring a portion of DNA from a resident ‘tumour inducing’ (Ti) plasmid into plant cells where it is integrated into a plant chromosome and expressed. This bacteria’s capacity for DNA transfer is the basis of most current plant genetic engineering and make it a model system for the study of pathogenic ...
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To improve Agrobacterium-mediated transformation of tobacco, factors influencing gene delivery, including genotype of the plant, bacterial strain, and Agrobacterium transformation procedure, were tested via direct somatic embryogenesis. Leaf tissue of three different tobacco genotypes (Nicotiana tabacum L. cvs. Samsun, and Xanthi, and N. benthamiana) we...
متن کاملAgrobacterium Mediated Transformation of Maize (Zea mays L.)
Agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. This gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. In the present work, we studied maize transformation using A. tumefaciens by identifying som...
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Members of the genus Agrobacterium cause the neoplastic diseases crown gall, hairy root, and cane gall on numerous plant species. Extensive genetic analyses conducted in the 1980s identified key bacterial genes involved in virulence. During the past decade, however, genomic technologies have revealed numerous additional bacterial genes that more subtly influence transformation. The results of t...
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ژورنال
عنوان ژورنال: Molecular Plant Pathology
سال: 2010
ISSN: 1464-6722,1364-3703
DOI: 10.1111/j.1364-3703.2010.00637.x