منابع مشابه
Agglutination of H. pertussis using concavity slides.
The agglutination reaction is the easiest to perform and is probably the most widely used of the serological tests for the detection of H. pertussis antibodies. Two methods have been used, a slide test and a tube test. A comparison of these techniques has been made by a number of workers (Evans and Maitland, 1939; Mishulow, 1942; Powell and Jamieson, 1942; Powell, 1944; and DeGara and Mayer, 19...
متن کاملFimbrial typing of Bordetello pertussis isolates: agglutination with polyclonal and monoclonal antisera.
As established by Eldering et al. (3), fimbrial antigens of Bordetella pertussis cells were used to raise agglutinating antibodies and have thus been called agglutinogens, which are constitutive parts of two fimbrial proteins, Fim2 and Fim3, and of an outer membrane protein called pertactin (Prn), respectively (1, 2). On the basis of this, monoclonal antibodies to Fim2 and Fim3 were obtained (4...
متن کاملSimple, speedy, sensitive, and specific serodiagnosis of pertussis by using a particle agglutination test.
We developed a particle agglutination test (KPA) with poly(gamma-methyl L-glutamate) as the solid particle for measurement of pertussis toxin (PT) antibody. In this study, KPA was assessed as a means of serodiagnosing pertussis, and the results were compared with those of indirect enzyme-linked immunosorbent assay (indirect ELISA) and the microagglutination test. First, four serum samples were ...
متن کاملFimbrial typing of Bordetella pertussis isolates: agglutination with polyclonal and monoclonal antibodies.
On the basis of serotyping 62 strains of Bordetella pertussis, Guiso and colleagues (N. Guiso, C. H. Wirsing von König, C. Becker, and H. Hallander, Letter, J. Clin. Microbiol. 39:1684– 1685, 2001) concluded that monoclonal and polyclonal reagents showed good concordance but that the source of the monoclonal antibodies needed to be specified. We have conducted a similar study on 209 strains of ...
متن کاملRegulation of pertussis toxin and lipopolysaccharide levels of Bordetella pertussis 134 in response to modulators
Whooping cough (pertussis) is a highly contagious disease of the human respiratory tract, which is caused by Bordetella pertussis. Reemerge of pertussis in some highly immunized populations and divergency in gene order among several B. pertussis strains promoted this research to study the change of pertussis toxin (PT) and lipopolysacharide levels in response to the different environments. Th...
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ژورنال
عنوان ژورنال: Archives of Internal Medicine
سال: 1916
ISSN: 0730-188X
DOI: 10.1001/archinte.1916.00080080101007