Age-related profile of beta-N-acetylhexosaminidase glycosylation in rat liver.
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چکیده
منابع مشابه
Age dependence of serum beta-N-acetylhexosaminidase (NAG) activity.
Serum N-acetyl-beta-D-glucosaminidase (NAG; EC 3.2.1.30) is a hexosaminidase and may be a predictor of vascular injury, e.g., in infant respiratory distress syndrome, pneumonia, broncho-pulmonary dysplasia and necrotizing enterocolitis. To estimate the new diagnostic prospects we have modified our urinary NAG assay. In this sensitive colorimetric micro-assay, VRA-GlcNAc was used as a substrate....
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beta-N-Acetylhexosaminidase I2 was purified from human liver by a combination of concanavalin A chromatography, DEAE-cellulose chromatography, gel filtration and affinity chromatography on 2-acetamido-N-(6-aminohexanoyl)-2-deoxy-beta-D-glucopyranosylamine coupled to CNBr-activated Sepharose 4B. Its specific activity was 130 mumol/min per mg of protein compared with values of 150 and 320 mumol/m...
متن کاملDistinguishing Candida species by beta-N-acetylhexosaminidase activity.
A variety of fungi produce the hydrolytic enzyme beta-N-acetylhexosaminidase (HexNAcase), which can be readily detected in assays by using p-nitrophenyl-N-acetyl-beta-D-glucosaminide as a substrate. In the present study we developed a microtiter plate-based HexNAcase assay for distinguishing Candida albicans and Candida dubliniensis strains from other yeast species. HexNAcase activity was detec...
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Lagnado, J. R. & Lyons, C. A. (1971) Biochem. J. 126, 9~-IOP Lagnado, J. R., Lyons, C. A. 8z Wickremasinghe, G. (1971) FEBSLett. IS, 254-258 Shelanski, M. L., Gaskin, F. &Cantor, C. R. (1973) Proc. Nat. Acad. Sci. U.S. 70,765-768 Tao, M., Salas, M. L. &Lipmann, F. (1970) Proc. Nat. Acad. Sci. US. 67,408-414 Weisenberg, R. C. & Timasheff, S. N. (1970) Biochemistry 9,4110-4116 Weisenberg, R. C., ...
متن کاملMolecular Phylogeny and Predicted 3D Structure of Plant beta-D-N-Acetylhexosaminidase
beta-D-N-Acetylhexosaminidase, a family 20 glycosyl hydrolase, catalyzes the removal of β-1,4-linked N-acetylhexosamine residues from oligosaccharides and their conjugates. We constructed phylogenetic tree of β-hexosaminidases to analyze the evolutionary history and predicted functions of plant hexosaminidases. Phylogenetic analysis reveals the complex history of evolution of plant β-hexosamini...
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ژورنال
عنوان ژورنال: Acta Biochimica Polonica
سال: 1998
ISSN: 1734-154X,0001-527X
DOI: 10.18388/abp.1998_4273