Agar media for tubercle bacilli

Semi-solid agar media for culture and drug sensitivity tests of tubercle bacilli from sputum.

mens were homogenized using a modified Petroff's method. Sputum was treated as described by Mackie and McCartney (1953) except that after removing the supernatant fluid subsequent to centrifuging for half an hour at 3,000 r.p.m. the neutralized deposit was centrifuged further for 5 min. at 1,000 r.p.m. This second spinning deposited the coarser particles of debris inseparable from sputum concen...

Selective media in the isolation of tubercle bacilli from tissues.

Direct culture on Lowenstein-Jensen slopes and on three media made selective for tubercle bacilli by the addition of four antibacterial agents was compared with guinea-pig inoculation on 490 tissue specimens. Tubercle bacilli were obtained from 15 specimens by culture and 14 by guinea-pig inoculation; only one specimen was positive by guinea-pig and not by culture. The most efficient culture me...

Factors Affecting the Growth of Tubercle Bacilli in Liquid Media

1. Certain water-soluble esters of long chain fatty acids (in particular of oleic acid) favor submerged and diffuse growth of mycobacteria throughout the depth of synthetic liquid media. 2. Esters of oleic acid increase considerably the amount of growth yielded by avian strains in synthetic media. 3. The addition of serum albumin to synthetic liquid media permits visible growth of minimal inocu...

Pyruvic acid egg medium for tubercle bacilli.

Sputum culture for tubercle bacilli on a solid egg medium containing glycerol was compared with culture on a similar medium containing pyruvic acid and no glycerol. Tubercle bacilli from the sputum of five out of 99 patients grew on pyruvic acid medium but not on glycerol medium. The addition of pyruvic acid is therefore essential when an egg medium is used for the cultural diagnosis of tubercu...

The Growth of Human Tubercle Bacilli, H37, in Synthetic Medium with and without Agar.