Agar diffusion test with ultraviolet-irradiated barley stripe mosaic virus
نویسندگان
چکیده
منابع مشابه
Comparison of barley stripe mosaic virus strains.
BSMV (barley stripe mosaic virus) particles were obtained in a pure state from infected host plant tissues of Hordeum vulgare. The three genomic parities (alpha, beta and gamma) were amplified by PCR using specific primers for each particle; each was cloned. Partial sequence of the alpha, beta and gamma segments was determined for the Egyptian isolate of barley stripe mosaic virus (BSMV AE1). A...
متن کاملMovement of a barley stripe mosaic virus chimera with a tobacco mosaic virus movement protein.
The tobacco mosaic virus (TMV) 30K movement protein (MP) gene was inserted into a full-length cDNA clone of barley stripe mosaic virus (BSMV) RNA beta replacing the triple gene block (TGB). The resulting recombinant ND-MPT genome, consisting of infectious wt transcripts of BSMV RNAs alpha and gamma, together with the hybrid RNA beta transcript, was inoculated onto test plants to study the funct...
متن کاملStructural Lability of Barley Stripe Mosaic Virus Virions
Virions of Barley stripe mosaic virus (BSMV) were neglected for more than thirty years after their basic properties were determined. In this paper, the physicochemical characteristics of BSMV virions and virion-derived viral capsid protein (CP) were analyzed, namely, the absorption and intrinsic fluorescence spectra, circular dichroism spectra, differential scanning calorimetry curves, and size...
متن کاملA Non-capsid Protein Associated with Unencapsidated Virus RNA in Barley Infected with Barley Stripe Mosaic Virus
متن کامل
Barley stripe mosaic virus and the frequency of triploids and aneuploids in barley.
BSMV infection caused a pronounced increase in the frequency of triploid and aneuploid seeds in eleven barley varieties, but with considerable variation in frequency among varieties. In some of the varieties triploids exceeded three per cent. In virus-free material a few triploids were found in most of the varieties, but the frequency was very low. There was, however, a significant variation am...
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ژورنال
عنوان ژورنال: Japanese Journal of Phytopathology
سال: 1965
ISSN: 1882-0484,0031-9473
DOI: 10.3186/jjphytopath.30.216