Affinity Separation: M13 Bacteriophage-Activated Superparamagnetic Beads for Affinity Separation (Small 15/2012)

Bacteriophage - Activated Superparamagnetic Beads for Affinity Separation

Protein separation using affinity-based reversed micelles

Reversed micellar two-phase extraction is a developing technique for protein separation. Introduction of an affinity ligand is considered to be an effective approach to increase the selectivity and capacity of reversed micelles. In this article, Cibacron Blue F3G-A (CB) as an affinity ligand was immobilized to reversed micelles composed of soybean lecithin by a two-phase reaction. The affinity ...

A Biotechnological Perspective on The Affinity Magnetic Separation and Purification Based on Oligonucleotides

The rapidly growing field of biotechnology has created a critical need for simple, fast andhigh-throughput processes for the separation and purification of biomolecules from biologicalmatrices. In recent years, several bioseparation techniques have been proposed as advancedalternatives to the classical separation methods. These modern processes emphasize ultrahighselective and sensitive analysi...

Advances in affinity ligand-functionalized nanomaterials for biomagnetic separation.

The downstream processing of proteins remains the most significant cost in protein production, and is largely attributed to rigorous chromatographic purification protocols, where the stringency of purity for biopharmaceutical products sometimes exceeds 99%. With an ever burgeoning biotechnology market, there is a constant demand for alternative purification methodologies, to ameliorate the depe...

Inertial Microfluidics for Multiplexed Affinity Separation of Proteins and Cells

We present an inertial microfluidic platform for rapid, multiplexed affinity purification of proteins and cells from complex mixtures. Beads of different sizes, coated with different bait molecules bind specific proteins or cells in a single reaction step and the bound targets are then size-sorted by flowing in a spiral microchannel. We use this scheme to simultaneously purify antibodies specif...