Affimer-based impedimetric biosensors for fibroblast growth factor receptor 3 (FGFR3): a novel tool for detection and surveillance of recurrent bladder cancer

Fibroblast growth factor receptor 3 (FGFR3) is a transmembrane tyrosine kinase protein in the fibroblast family, which plays key role many biological processes. Over-expression and activating mutations FGFR3 are frequent non-invasive bladder cancer, highlighting this as potential biomarker for recurrent cancer detection. Affimer reagents isolated against recombinant were assessed their affinity using double-sandwich ELISA SPR. Anti-FGFR3-14 FGFR3-21 proteins showed strong binding to used fabrication of impedimetric electrochemical biosensors. A decrease impedance was observed when sensors exposed increasing concentrations FGFR3. The successful biosensors capable detecting sub-pM nM phosphate buffered saline well synthetic urine.